Quantification of carboxyl groups in carbodiimide cross-linked collagen sponges
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چکیده
منابع مشابه
Quantification of carboxyl groups in carbodiimide cross-linked collagen sponges.
Glutaraldehyde (GA) fixation of bioprosthetic tissue is a well adapted technique, with commercial products on the market for almost 40 years. Amine groups present in tissue react with GA to form different types of cross-links. An estimation of the degree of cross-linking of the tissue can be obtained by measuring the concentration of residual amine groups, which is frequently carried out with t...
متن کاملIn vitro degradation of dermal sheep collagen cross-linked using a water-soluble carbodiimide.
Bacterial collagenase was used to study the susceptibility of dermal sheep collagen (DSC) cross-linked with a mixture of the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide hydrochloride and N-hydroxysuccinimide (E/N-DSC) towards enzymatic degradation. Contrary to non-cross-linked DSC (N-DSC), which had a rate of weight-loss of 18.1% per hour upon degradation, no weig...
متن کاملFunctional carboxyl groups in the red cell anion exchange protein. Modification with an impermeant carbodiimide
Anion exchange in human red blood cell membranes was inactivated using the impermeant carbodiimide 1-ethyl-3-(4-azonia-4,4-dimethylpentyl)-carbodiimide (EAC). The inactivation time course was biphasic: at 30 mM EAC, approximately 50% of the exchange capacity was inactivated within approximately 15 min; this was followed by a phase in which irreversible exchange inactivation was approximately 10...
متن کاملEffect of Solvents on Dentin Collagen Cross-linking Potential of Carbodiimide.
PURPOSE This study compared the dentin collagen cross-linking potential of carbodiimide (EDC) prepared in three most commonly used solvents in dental adhesive systems: water, ethanol, and acetone. MATERIALS AND METHODS Thirty-eight extracted caries-free human permanent molars were used in this study. Demineralized dentin beams were prepared and cross linked by 0.3 M EDC in water, acetone, or ...
متن کاملCross-linking of dermal sheep collagen using a water-soluble carbodiimide.
A cross-linking method for collagen-based biomaterials was developed using the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC). Cross-linking using EDC involves the activation of carboxylic acid groups to give O-acylisourea groups, which form cross-links after reaction with free amine groups. Treatment of dermal sheep collagen (DSC) with EDC (E-DSC)...
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ژورنال
عنوان ژورنال: Journal of Biomedical Materials Research Part A
سال: 2007
ISSN: 1549-3296,1552-4965
DOI: 10.1002/jbm.a.31398